Extended Data Fig. 1: Multiplexed sgDNase pretreatment. | Nature Biomedical Engineering

Extended Data Fig. 1: Multiplexed sgDNase pretreatment.

From: Detection of low-frequency mutations in clinical samples by increasing mutation abundance via the excision of wild-type sequences

Extended Data Fig. 1

Next-generation sequencing results of the five EGFR and KRAS mutations (EGFR L858R, EGFR 19del, EGFR G719S, EGFR T790M and KRAS G13D) in mixed synthetic samples before (a1-a5) and after (b1-b5) 5-plex sgDNase pretreatment. The initial mutation abundance was 0.4% for EGFR G719S and 0.1% for other tested mutation. The five target mutations were pretreated by sgDNase simultaneously. The variant to noise plots derived from Illumina MiSeq sequencing data are depicted.

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