Extended Data Fig. 2: FEMMAN workflow at the molecular scale, and titration curve for SARS-CoV-2 detection.
a, IRDye800 fluorescence images of a typical titration curve for a 22 nt DNA target (Target A in Supplementary Table 4) detection from 2 nM to 2 fM on pGOLD substrate, glass substrate and evaporated Au film with Probe A (Supplementary Table 2). b, Fluorescence quantification of DNA titration on the three substrates for 4 DNA sequences (Probe A, B, C & D in Supplementary Table 2, Target A, B, C & D in Supplementary Table 4). c, Detailed molecular schematic of SARS-CoV-2 viral RNA detection using FEMMAN assay. d, Sequences of the DNA probes and primers for N501 target site. The target site is highlighted in green. e, IRDye800 fluorescence images of a typical titration curve for SARS-CoV-2 wild type viral RNA detection by FEMMAN from 5,000 copies/reaction to 5 copies/rxn on pGOLD and glass substrate. f, Background subtracted fluorescence quantification of viral RNA titration on pGOLD for N501N, N501Y and NTC probes. n = 3 technical replicates, bars represent mean ± s.d.
