Fig. 3: Images of a transgenic mouse brain that expresses GCaMP6f in neurons. | Nature Biomedical Engineering

Fig. 3: Images of a transgenic mouse brain that expresses GCaMP6f in neurons.

From: Multifocal fluorescence video-rate imaging of centimetre-wide arbitrarily shaped brain surfaces at micrometric resolution

Fig. 3

a, The standard deviation of neural activity captured through MFIAS macroscopy and aligned with the Allen Mouse Brain Common Coordinate Framework. Four zoomed-in views are displayed on the right. Scale bars, 1 mm (global view) and 100 μm (local views). b, In the calibration stage, the mouse brain was axially scanned with different glass thicknesses. The neural position of each image depth was detected. Different colours in the image represent different glass thicknesses. Scale bar, 1 mm. c, The activities of 737 neurons from the four zoomed-in regions shown in a, sorted by Z-score. The right panel displays the traces of randomly selected neurons sorted from high to low Z-scores. Neural signals were detected in both central and corner regions using MFIAS macroscopy (sample size n = 6 mice). d, The distribution of PSNR for all sources across the FOV by MFIAS. e, The PSNR distribution for the same sources by a conventional microscope. The PSNR for the neural signal is almost constant for MFIAS but drops to the baseline at the lateral edge regions for the conventional microscope. d and e are representative micrographs out of three biological replicates obtained (source numbers 302, 337 and 668, in the 1,000-frame sequences), and the additional micrographs are included in Supplementary Note 6. Central black mark: median for neural sources. Bottom and top edges: 25th and 75th percentiles. Whiskers extend to extreme points excluding outliers (1.5 times above or below the interquartile range). Scale bars, 1 mm. f, Visual stimulus of drifting gratings was performed on the mouse, and visually responsive neurons in the visual cortex were detected in three mice using one-way ANOVA (P < 0.01). Blue circles, visually responsive but OSI <0.8 neurons. Red circles, OSI ≥0.8 neurons. g, The neural responses of four typical high OSI neurons in different brain areas. Error bars represent the trimmed standard deviation of the five trials. h, Traces of the neurons in g. Colour stripes in the figure represent periodic visual stimulus moments with angles of 270°, 135°, 315°, 225°, 0°, 180°, 45° and 90°. e and g are representative micrographs out of three biological replicates obtained, and the additional micrographs are included in Supplementary Note 6.

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