Extended Data Fig. 4: By trichrome staining, ratio of blue to red increases stepwise from non-DSD to DSD to ASD.
A, Representative images of LF from non-DSD, DSD, and ASD patients stained with trichrome staining. B, Representative images of the color thresholding protocol for quantification. Percent blue and red was quantified using a standard color thresholding protocol for all images; red pixels matching a set range of values were selected (top, selected pixels highlighted red). The remainder of pixels were considered blue and the images were converted to binary images for quantification (bottom, white=red and black=blue). C, The ratio of blue to red significantly increases from non-DSD (n = 2) to DSD (n = 3, p = 0.022) and increases again from DSD to ASD (n = 3). Quantitative data are shown as mean ± sem, all tests are two-sided, unpaired t-tests.
