Extended Data Fig. 4: Intensity quantification of NUCB1 and Lgr3 antibodies immunostaining.
a, NUCB1 antibody specificity was tested by the NUCB1 inhibition using heat-shock Gal4 (Hs > NUCB1-RNAi). b, Staining intensity of neurons was measured with LSM 5 software program by drawing a dotted line as shown. c-e, The staining intensity of neuronal cells in Fig. 4 was quantified using LSM 5 software program and illustrated as intensity graphs. Control (n = 5), GMR > ykiSA (n = 10), GMR > ykiSA, Lgr3Δ50 (n = 5). f-g, A subset of sNPF (f) and NPF (g) expressing neurons in the cancer condition (GMR > ykiSA) were overlapped with NUCB1 expressing neurons. Scale bars are 100 μm. Statistical source data and unprocessed western blots.
