Extended Data Fig. 10: CSNK1A1 haploinsufficiency sensitizes human CD34+ cells to LEN. | Nature Cell Biology

Extended Data Fig. 10: CSNK1A1 haploinsufficiency sensitizes human CD34+ cells to LEN.

From: Loss of lenalidomide-induced megakaryocytic differentiation leads to therapy resistance in del(5q) myelodysplastic syndrome

Extended Data Fig. 10

Human cord blood CD34+ cells were transduced with shRNA targeting CSNK1A1 or empty vector control and treated with LEN (1 µM) or DMSO for 3 days. a, Gating strategy for the analysis of cord blood cells. b, Analysis by flow cytometry showed increased Annexin V+ staining of LEN-treated shCSNK1A1 cells compared to shControl. (n = 6 independent experiments) c–e, Percentage of CD11b+ (c), CD14+ (d) or CD235a+ (e) cells within the apoptotic (Annexin V+) compartment was not altered by treatment with LEN in shCSNK1A1 cells (n = 3 independent experiments). f, Representative flow panel (left) showing apoptosis (Annexin V+ cells) occurring mainly in the CD34- compartment in shCSNK1A1 cells after 3-day treatment with LEN (1 µM) or DMSO. Percentage of Annexin V+ cells (right) in the CD34+ and CD34- compartments in shCSNK1A1 cells after 3 days of LEN (1 µM) or DMSO treatment (n = 3 independent experiments). Statistical analysis was performed using ANOVA with multiple correction adjustment (Bonferroni). Data is presented as mean ± s.d. Source data are available online.

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