Extended Data Fig. 9: Both Arg4 and Lys7 of RAB22A-NeoF1 are required for the functions of RAB22A-NeoF1.

a, g, Quantification analyses of adhesion of the indicated cells. Mean ± s.d. of n = 3 biologically independent experiments, p values are shown. Two-tailed Student’s t-test. Vector, vector-only control. b–d, h–j, The orthotopic osteosarcoma metastasis model in vivo using the indicated stable cell lines (n=6 biologically independent animals). b, h, Quantification analyses of wet lung weights. Data are the mean ± s.d. p values are shown. A two-tailed Student’s t-test was performed. c, d, i, j, Kaplan-Meier survival data were plotted as the percent of mice surviving in the indicated groups using a predefined cutoff tumor volume of 1.5 cm³. p values are shown. The Log-rank test was performed. Vector, vector-only control. e, Ambience of key residues of R4 and K7 in RAB22A. RAB22A is shown as a cartoon representation with residues 1-10 colored cyan. R4, K7, and the residues surrounding K7 are shown as ball-and-stick models. f, 293T cells transfected with the indicated plasmids were lysed and subjected to immunoprecipitation using streptomycin beads and then eluted with biotin. The eluates were incubated with the purified SmgGDS607-His protein and the immunoprecipitated complex using a nickel column followed by Western blotting. Data are representatives of n = 3 biologically independent experiments. Statistical source data are provided in Source Data Extended Data Fig. 9.

Source data