Extended Data Fig. 5: H3.3 maintains HSC repopulation in a competitive setting.
a. Representative flow cytometric plots for the CD45.2+ LinN cells and LT-HSC at week 16 following tamoxifen injection. b. The percentage and total number of CD45.2+ LinN cells within the BM of recipient mice. c. The percentages of CD45.2+ cells within the BM, spleen, and thymus of recipient mice. d-e. The total numbers of stem and progenitor cells, and lineage positive cells within the BM of recipient mice. f-g. Representative flow cytometric plots for the lineage cells (CD11b+, CD11b+Gr1+, Gr1+, or B220+ cells) within the BM or spleen of recipient mice. h. Representative flow cytometric plots for lineage cells (CD4+, CD8+, or CD4+CD8+ double positive cells) within thymus or spleen. i-j. The percentages of lineage-specific cells within BM or spleen of the recipients. k-l, The percentages of lineage cells within thymus or spleen of the recipients. For all the panels, n=6 animals for AKOBhet group; n=6 animals for DKO group. Error bars represent standard error of the mean. p-value is calculated using unpaired, 2-tailed t-test.
