Extended Data Fig. 2: SEpro validation using an in vitro developmental model.
From: Single-cell atlas of human liver development reveals pathways directing hepatic cell fates
a, PCA analysis of hiPSC-derived hepatic stellate cells (HSLC) and hiPSC-derived endothelial cells (ELC) confirming their common bipotent stellate-endothelial progenitor (SEpro) stage following mesoderm induction and before cell lineage specification (scRNA-seq integrates n = 5 differentiation timepoints). b, UMAP visualization (30 neighbours, 500 PCs) demonstrating the highly correlative relationship of SEpros to the transcriptomically similar ELC-D3.5 and HSLC-D7 stages of in vitro differentiation. c, Diffusion pseudotime confirming the specification of foetal-like HSLCs and ELCs from their common bipotent progenitor. d, UMAP visualizations showing the co-expression of key mesenchymal and endothelial markers such as CDX1, PDGFRB and KDR during the in vitro SEpro stage, followed by upregulation of lineage-specific markers and loss of co-expression. e, QPCR analyses of hiPSC differentiated first toward endothelial cells, then transitioned into culture conditions to specify hepatic stellate cells upon reaching the bipotential SEpro stage. These qPCR analyses show the acquisition of hepatic stellate cells markers and f, the loss of endothelial markers (n = 2 independent cell lines). Data are presented as mean values + /- SEM. Feature plot colour scales show “gene expression [log-normalized, scaled counts]”.
