Extended Data Fig. 10: The expression and role of PD-L1 for T-ALL.
a,b, Representative imaging of GFP+Tomato+ (PD-1+ cells), GFP+Tomato– (PD-1–) T-ALL cells, and PD-L1+ cells were analyzed at d1 after tamoxifen injection in T-ALL mice engrafted with Pd-1-CreERT2; tdTomato T-ALL cells at 3 weeks after transplantation. The yellow arrows indicated representative PD-L1+ T-ALL cells (GFP+), PD-L1+ normal T cells (GFP–CD3+), PD-L1+ myeloid cells (CD11b+), PD-L1+ endothelial cells (ECs, Endomucin+) (a), and PD-1+ T-ALL cells (Tomato+GFP+) (b). The scale bar was 5 µm. c, Quantification of the distance between PD-1–, PD-1+ T-ALL cells, and randomly placed spots with PD-L1 expressing cells in the bone marrow of T-ALL mice cells at 3 weeks after transplantation (n = 3 mice). d, Quantification for Fig. 6l (d) (n = 3 mice). e, Schematic depicting strategy of using anti-CD3/CD28 antibodies to stimulate TCR signaling in Pd-l1 deleted PD-1+ and PD-1– T-ALL cells (left) and western blots of indicated proteins in Pd-l1 deleted PD-1– or PD-1+ T-ALL cells with indicated treatments for 72 h (right). Similar blots were obtained in three independent experiments. f, Quantification for Fig. 6o (n = 3 mice). The P values in c,d,f were determined by a two-way analysis of variance (ANOVA). All data are shown as mean ± s.d.
