Extended Data Fig. 6: STING co-localized with Rab11 within lysosomes. | Nature Cell Biology

Extended Data Fig. 6: STING co-localized with Rab11 within lysosomes.

From: STING signalling is terminated through ESCRT-dependent microautophagy of vesicles originating from recycling endosomes

Extended Data Fig. 6

a, Sting−/− MEFs stably expressing mRuby3-STING (magenta), Lamp1-EGFP (cyan) were treated with or without HaloTag SaraFluor 650 T ligand (yellow) (the left 4 panels). Sting−/− MEFs stably expressing mRuby3-STING (magenta), Lamp1-EGFP (cyan), and Halo-Rab11a (yellow) were treated with or without HaloTag SaraFluor 650 T ligand (the right 4 panels). Scale bars, 10 µm. b, The far-red fluorescence intensity of the cells was quantified. Data are presented in box-and-whisker plots with the minimum, maximum, sample median, and first vs. third quartiles. c, Sting−/− MEFs stably expressing mRuby3-STING (magenta), Lamp1-EGFP (cyan), and Halo-Rab11a (green) were treated with DMXAA for 6 h in the presence of E64d/pepstatin A/orlistat. Scale bars, 500 nm. The sample size (n) represents the number of cells (b). Source numerical data are available in source data.

Source data

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