Extended Data Fig. 1: Histology and dissection images reveals early dynamics of early germ layers of rabbit flat-disk embryo.
From: An atlas of rabbit development as a model for single-cell comparative genomics
a) Representative RNAscope image of GD7 rabbit sagittal in utero section showing beginning of gastrulation. TFAP2C and OCT4 mark trophoblast and epiblast-derived cell types, respectively. Arrowheads indicate implantation on the anti-mesometrial side of the embryo. b) Representative RNAscope image of GD8 rabbit sagittal in utero sections showing germ layer differentiation. (i) Arrowheads indicate implantation sides in the mesometrial direction. TFAP2C marks trophoblast and trophoblast progenitors. (ii) Chorioamnion fold formation can be seen alongside early germ layer differentiation. Chorion consists of a mesoderm inner layer, and amnion has an mesoderm outer layer as captured in the RNAscope image. c) Representative histology images of rabbit cross-sections in utero and magnification of the embryo to show details and orientation of the embryo in the uterus. cm, cranial mesoderm. d) Summary of over 650 + H&E and RNAscope images of rabbit sections generated in this study of which the representative images are pulled from. e) RNAscope image of the vitelline artery (VA) and dorsal aorta (DA) region showing the RUNX1 + CDH5+ presumptive haemogenic endothelium in the ventral portion of the dorsal aorta. f) Representative images of the rabbit embryos and their extraembryonic tissues utilized in this study g) 13 somite and 16 somite GD9 rabbit embryos microdissected into anterior, mid, and posterior regions for scRNA-seq.
