Extended Data Fig. 7: STING stable expression in MC38 cells promotes antitumour immunity.
From: STING is a cell-intrinsic metabolic checkpoint restricting aerobic glycolysis by targeting HK2
a, WCLs from L929, 293T, MC38, CT26 were analysed by immunoblotting. b, Representative MC38 tumour staining of Ki-67 and cleaved caspase-3 as described in Fig. 5b. The percentage of Ki-67- or cleaved caspase-3-positive cells was quantified (n = 6). Scale bars, 200 μm. c, Flow cytometry analysis of lymphocyte in MC38 tumours as described in Fig. 5b (n = 8). d, Flow cytometry analysis of tumour-infiltrating CD4+ T cells and the severely exhausted CD4+ cells in MC38 tumours as described in Fig. 5e (n = 4 Vector group, n = 5 STING group). e and f, Flow cytometry analysis of CD8+ and CD4+ T cells in the spleens of the MC38 tumour-bearing mice as described in Fig. 5e (n = 4 Vector group, n = 5 STING group). g, Representative MC38 tumour-bearing in NSG mice staining of Ki-67 and cleaved caspase-3 as described in Fig. 5f. The percentage of Ki-67- or cleaved caspase-3-positive cells was quantified (n = 6). Scale bars, 200 μm. Data are presented as means ± s.d., and statistical analyses were performed using a two-tailed unpaired Student’s t-test. Data are representative of three independent experiments. Source numerical data and unprocessed blots are provided in Source Data.
