Extended Data Fig. 1: STING restricts aerobic glycolysis.
From: STING is a cell-intrinsic metabolic checkpoint restricting aerobic glycolysis by targeting HK2
a, Image of the colour of the cell culture media. b, the number of control or Sting1−/− L929 cells was quantified at the indicated time points. c, qPCR analysis of Isg56 or Cxcl10 mRNA in control or Sting1−/− L929 cells transfected with or without HT-DNA. d and e, immunoblotting analysis of L929 cells transfected with HT-DNA or infected with HSV-1. f, L929 cells were pre-treated with chloroquine (20 μM) or bafilomycin A1 (400 nM) for 30 minutes and then transfected with HT-DNA (1 μg/ml), followed by immunoblotting. g, L929 cells were infected with HSV-1 (MOI = 1) and treated with chloroquine (20 μM) or bafilomycin A1 (400 nM). WCLs were analysed by immunoblotting. h, L929 cells were pre-treated with chloroquine (20 μM) or bafilomycin A1 (400 nM) for 30 minutes and then transfected with HT-DNA (1 μg/ml). The expression of Cxcl10 and Isg56 was quantified at 8 h post-transfection. i, L929 cells were pre-treated with chloroquine (20 μM) or bafilomycin A1 (400 nM) for 30 minutes and then transfected with HT-DNA (1 μg/ml), followed by lactate meaturement. j, L929 cells were infected with HSV-1 (MOI = 1) and treated with chloroquine (20 μM) or bafilomycin A1 (400 nM). Lactate was measured at the indicated time points. k, L929 cells were pre-treated with brefeldin A (10 μg/ml) for 30 minutes and then transfected with HT-DNA (1 μg/ml). The expression of Cxcl10 and Isg56 was quantified at 8 h post-transfection. l, L929 cells were transfected with HT-DNA (1 μg/ml) and treated with brefeldin A (10 μg/ml). lactate was measured at 24 h post-transfection. Data are presented as means ± s.d. of n = 3 independent biological replicates for b, c, h-l. P-value was calculated by two-tailed Student’s t-test. Data are representative of three independent experiments. Uncropped gel images and numerical data are available in source data.
