Extended Data Fig. 8: FGF signalling regulates ERK and ETV4.
From: ETV4 is a mechanical transducer linking cell crowding dynamics to lineage specification
a, FPKM of EGFR and FGFRs in undifferentiated H9 hESCs (GSE183702). n=4 samples pooled from four independent experiments. b,c, Validation of lentiviral vectors expressing FGFR1 shRNAs in H9 hESCs by qPCR (b) and immunostaining (c). n=3 samples for qPCR (b) and n=33 cells for immunostaining (c). d, Immunofluorescence assay for pERK in H9 hESCs transduced with lentiviral vectors expressing FGFR1 shRNAs together with GFP. n=50 cells pooled from two independent experiments. e, Immunofluorescence assay for ETV4 in H9 hESCs transduced with lentiviral vectors expressing FGFR1 shRNAs together with GFP. n=37 cells pooled from two independent experiments. f, Immunofluorescence assay for ETV4 in small and large H9 colonies cultured in a transwell system. n=30 cells. n = number of cells (c and f) pooled from three independent experiments. Two-sided Student’s t-test, ***P < 0.001, **P < 0.01, *P < 0.05. Exact P values are presented in Supplementary Table 9. Scale bars: 25 µm (c,d,e, and f). Numerical source data are available in Source data.
