Extended Data Fig. 5: Tie-B promotes the systemic accumulation of exhaustion-like T cells.
From: Solid tumour-induced systemic immunosuppression involves dichotomous myeloid–B cell interactions
a, b, Tumor cell numbers four days after cocultured with Tie-B cells. n = 3 independent repeats. Unpaired two-tailed Student’s t-test, mean ± s.d. c, PyMT-M tumor growth curves during T-cell transfer and ICB treatment. Unpaired two-tailed Student’s t-test on day 20. d, PD-1+CTLA-4+ T cells in 2208L-bearing mice after B cell depletion. n = 5 mice per group. One-way ANOVA with Tukey’s multiple comparisons test (left panel). The line inside the box is the median value, and the bottom/top bars of the box indicate min to max values. e, B220+ cells and PD-1+CTLA-4+ T cells in naïve mice after B cell depletion. n = 5 mice. Unpaired two-tailed Student’s t-test, mean ± s.d. f, Mean fluorescent intensity (MFI) of ID2 protein in T cells from naïve or TiBA-2 tumor-bearing mice. n = 4 mice. Unpaired two-tailed Student’s t-test, mean ± s.d. g, PD1+CTLA4+ proportions after CD8T cells were co-cultured with Tie-B cells. n = 3 biological replicates. Unpaired two-tailed Student’s t-test, mean ± s.d. h, The expression level of MHC-II genes in B cell clusters based on the scRNA-seq data. i, j, MFI of MHC-II protein in B cell subsets in TiBA-2 tumor-bearing mice. n = 10 mice. Two-way ANOVA with Dunnet’s multiple comparisons test (i) and one-way ANOVA with Tukey’s multiple comparisons test (j), mean ± s.d. k, l, Tie-B cells were stimulated with ovalbumin323-339 peptides, co-cultured with OT-II CD4+ T cells, then detected with MHC-II-OVA-tetramer antibodies. n = 3 biological replicates, unpaired two-tailed Student’s t-test, mean ± s.d. DC, dendritic cells (positive control) m, Number of migrated T cells. n = 3 biological replicates, unpaired two-tailed Student’s t-test, mean ± s.d. n, Representative IF staining images of Tie-B cells (B220+CD93+) and CD8 T cells in spleen. Yellow arrows indicate aggregations. Representative of three independent experiments. o, Establishment of tumor-bearing Cd79a-CreER;Hmgb1fl/fl mice for tamoxifen treatment. p, PD1+CTLA4+ proportions after co-culture of CD3T and Tie-B cells. n = 3 biological replicates. One-way ANOVA with Dunnet’s multiple comparisons test, mean ± s.d. q, MFI of ID2 protein in T cells after co-culturing with Tie-B. n = 3 biological replicates. One-way ANOVA with Dunnet’s multiple comparisons test, mean ± s.d.
