Extended Data Fig. 6: TiBA-1/2 cannot be induced solely by direct interactions between cancer cells and early B cells.
From: Solid tumour-induced systemic immunosuppression involves dichotomous myeloid–B cell interactions
a, Flow cytometry analysis of myeloid cell numbers in E0771 tumors from the experiment in Fig. 4t, u. n = 5 mice/group. b–e, Flow cytometry analysis of myeloid cell subsets in the PB, spleen, BM and tumors of PyMT-N tumor-bearing Cd79a-CreER or Cd79a-CreER;Hmgb1fl/fl mice after tamoxifen treatment. n = 4 mice/group. Unpaired two-tailed Student’s t-test, mean ± s.d. f–h, Flow cytometry analysis of B cell subsets in the PB, spleen and BM of PyMT-N tumor-bearing Cd79a-CreER or Cd79a-CreER;Hmgb1fl/fl mice after tamoxifen treatment. n = 4 mice/group. Unpaired two-tailed Student’s t-test, mean ± s.d. i, Diagram showing co-culture of TiBA-1 or TiBA-2 tumor cells with early B cells in a non-direct contact manner to mimic the remote effect of cancer cells. j, k, Flow cytometry analysis of the absolute cell numbers of early B cell subsets 2 days after co-culture with TiBA-1 (LLC) or TiBA-2 (PyMT-N) tumor cells. n = 3 (B cells were isolated from 3 biological replicate mice). Two-way ANOVA with Dunnett’s multiple comparisons test compared to “B+NC” group, mean ± s.d. l, Diagram illustrates the 2208 L (TiBA-1) tumor-bearing CD11b-DTR or WT mice that received DT treatment. m, Flow cytometry analysis of absolute cell numbers of mature B cells (Fr. F) in the BM of 2208L tumor-bearing WT or CD11b-DTR mice after DT treatment (day 21). n = 4 mice/group. The line inside the box is the median value, and the bottom/top bars of the box indicate min to max values. n, Feature plot showing the CD11b expression in BM cell clusters. o, Representative flow cytometry images of CD11b and B220 expression in the BM of 2208L tumor-bearing mice.
