Extended Data Fig. 6: Hexanediol abolishes Rad52 and Mre11-GFP foci and cohesin-dependent tethering in absence of MRX.
From: Cohesin complex oligomerization maintains end-tethering at DNA double-strand breaks
a–c, Representative images of Rad52-YFP (a) and Mre11-GFP (c) foci at 2 h DSB with no treatment (DSB), or 30 minutes digitonin + hexanediol (DSB Hex) treatment. The images are maximum intensity projections and are representative of cells quantified in b–d. Scale bars, 1 µm. b–d, Quantification of cells with Rad52-YFP (b) and Mre11-GFP (d) foci at 2 h DSB with no treatment (DSB), or 30 minutes digitonin + hexanediol (DSB Hex) treatment. Data represent the analysis of N = 188 (b, DSB), N = 146 (b, DSB Hex), N = 97 (d, DSB) and 126 (d, DSB Hex) cells. e, f, Scatter dot plots of end separation corresponding to the pool of measurements of biological replicates presented in Fig. 4c,d. Red line at median. Source numerical data are available in Source data.
