Extended Data Fig. 1: In vivo assembly of a ternary Integrator module in human neuronal cells.
From: The enhancer module of Integrator controls cell identity and early neural fate commitment
(a) Heatmap clustering of all Integrator subunits (RNA expression levels=normalized transcripts per million bases, nTPM) across the full collection of non-neuronal human tissues and cell lines from the Human Protein Atlas project (proteinatlas.org). INTS10 expression is not increased across all tissues. (b) The number of INTS10, INTS13, and INTS14 peptides pulled down with GFP-FLAG, INTS10-FLAG, INTS13-FLAG, and INTS14-FLAG IPs in the nuclear extract of HEK293T cells stably overexpressing GFP-FLAG, INTS10-FLAG, INTS13-FLAG or INTS14-FLAG. (c) Immunoblot analysis of INTS10 IP from the nuclear extract of SH-SY5Y WT cells. Endogenous INTS10 co-precipitates along with other Integrator subunits (INTS1, INTS5 and INTS13). Two independent experiments yielded consistent results.(d) Endogenous INTS10 and INTS13 nuclear interactomes in parental SH-SY5Y cells. IP were performed using commercially available antibodies, followed by MudPIT LC-MS/MS analysis. Log10 iBAQ protein scores of each interactor are plotted. (e) Endogenous INTS5 and INTS10 nuclear interactomes in parental SH-SY5Y cells. IP were performed using commercially available antibodies, followed by MudPIT LC-MS/MS analysis. Log10 iBAQ protein scores of each interactor are plotted. Source numerical data and unprocessed blots are available in source data.
