Fig. 6: pNSCs are composed of Sox1⁺ cells.

a,b, Uniform manifold approximation and projection (UMAP) of 4,640 adult brain (a) and 5,981 lung (b) Nes-GFP⁺ cells. Colours represent cell population clusters. n = 1 biological replicate. c, FACS strategy for GFP⁺ and GFP⁻ cells from postnatal Sox2-GFP mouse lung tissue and morphologies of a primary lung pNSC cluster and GFP⁻ cells after culture. d, Sox2-GFP⁺ lung pNSCs stably maintained for 50 passages. e, Immunofluorescence of brain NSCs and lung pNSCs from Sox2-GFP mouse. Scale bar, 50 μm. f, Heatmap of global gene expression pattern across samples. n = 3 biological replicates for brain NSCs and lung pNSCs. n = 2 biological replicates for lung cells. All replicates are shown. g, Hierarchical clustering of the cell lines based on f. h, UMAP of 28,170 postnatal lung Sox2-GFP⁺ cells. Colours represent cell population clusters. n = 1 biological replicate. i, Expression of Sox1 and Sox2 in postnatal lung Sox2-GFP⁺ neural cells cluster. j,k, Immunohistochemistry of WT postnatal lung (j) and tail (k) tissues. Data represent three biological replicates. Scale bar, 50 μm. l,m, FACS strategy for deriving lung (l) and tail pNSCs (m) from postnatal Sox1-GFP mouse, and morphologies of primary lung (l) and tail pNSC cluster (m) and GFP⁻ cells after culture.