Extended Data Fig. 1: Merger of CuPDF, CuAAC and SuFEx for synthesis of a non-uniform, sequence-defined polymer.
From: Click processes orthogonal to CuAAC and SuFEx forge selectively modifiable fluorescent linkers
As an example, subjection of two-pronged monomers iii and iv on the one hand, and v and vi on the other, to the envisioned link-and-in situ modify conditions would deliver complementary dimers VII and IX (via VI and VIII, respectively). While VII would contain a fluorosulfate and a terminal alkyne, IX would be equipped with an azide (for CuAAC, in red) and a silyl ether (for SuFEx, in brown). Simultaneous subjection of an equal mixture of VII and IX to copper and base catalysts needed for CuAAC and SuFEx, respectively, would afford sequence-defined polymer X with amendable and differentiable linkers, depending on the amino alcohols involved in the modify steps (namely, G1 and G2). Hence, after just four chemical steps, performed in three vessels, and without the need for protection/deprotection or any other type of functional group adjustments, four monomers iii-vi could be transformed to polymer X. CuAAC, Cu-catalysed azide–alkyne cycloaddition; SuFEx, sulfur-fluoride exchange; M, monomer.
