Extended Data Fig. 3: Release of CDN from the PEG-lipid prodrug in cells.

a, Schematic of the chemistry of enzymatic cleavage of the dipeptide linker and subsequent self-immolative linker reaction. b, RAW macrophages were incubated for 18 hr with parent CDN or LND-CDN at varying concentrations. Cells were washed, then lysed and lysates were probed for quantity of parent CDN recovered by liquid chromatograph MS/MS analysis. c, Human THP-1-ISG reporter cells were incubated with indicated concentrations of LND-CDN for 3 hr, washed into fresh medium, then cultured for an additional 21 hr, followed by measurement of interferon-stimulated gene reporter activation by luciferase expression (n = 2 independent biological samples for each concentration and mean±s.e.m. is plotted; RLU, relative light units).