Extended Data Fig. 7: E. coli PBP2 variants co-purify with E. coli RodA. | Nature Microbiology

Extended Data Fig. 7: E. coli PBP2 variants co-purify with E. coli RodA.

From: Structural coordination of polymerization and crosslinking by a SEDS–bPBP peptidoglycan synthase complex

Extended Data Fig. 7

PBP2 variants were co-expressed with FLAG-tagged RodA and purified using anti-FLAG affinity resin. SDS-PAGE gel showing the elution from the anti-FLAG affinity resin demonstrates that PBP2 remains associated with RodA throughout the purification. Low (L) and high (H) correspond to approximately 2 µg and 4 µg as measured by A280. Results are derived from one experiment.

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