Extended Data Fig. 2: Strain-level in vitro and in vivo assessment of C. perfringens virulence potentials.
a, Oxygen tolerance test. Comparison of viability of C. perfringens strains (n = 31) from lineages I, III, V, VI and VII exposed to ambient air (aerobic) over 14 days in 3 biologically independent samples for each strain. Cell viability in percentage was shown from 48 h to 192 h between lineage VII and lineage V with statistical difference. The box in boxplot represents 50% of the central data, in between lower and upper quartile, with a central line representing the median while whiskers show the most extreme data points (also in b). b, Intestinal colonisation of C. perfringens monitored over 7 days post-challenge. LD, limit of detection. c, NMDS plot of murine genus-level gut microbiome profiles on day 0, day 2 and day 6. d, Comparison of genus-level murine gut microbiome on day 0, day 2 and day 6. A total of 4 groups were compared, Control (n = 5), ABX (n = 5), pfoA+ (n = 15) and pfoA- (n = 15). DPC: days post-challenge. (All statistical analyses were performed with Kruskal-Wallis test and post-hoc Dunn’s test of multiple comparisons with P values adjusted by Benjamini-Hochberg method. The box in boxplots represents 50% of the central data, in between first and third quartile, with an inner line representing the median).
