Extended Data Fig. 1: Effect of D-amino acids in motility.
From: d-amino acids signal a stress-dependent run-away response in Vibrio cholerae
a, Motility analysis of V. cholerae in soft agar in presence of L- and D-amino acids. Grey bars indicate relative motility of BsrV knockout mutant compared to wild-type (wt) both in presence and absence of 5 mM amino acids. b, Effect of D-amino acids (DAAs) in growth. Survival rate was calculated as relative maximum growth in late stationary phase using the not supplemented culture as reference. Cultures were supplemented with 5 mM DAAs. c, Spot assay for the indicated V. cholerae strains. Cells were grown in TB until stationary phase, serially diluted and spotted on LB agar plates supplemented with 5 mM DAAs. Error bars in a and b represent mean values ± SD of 6 and 3 biologically independent replicates. Significant differences (One-way ANOVA, p-values were adjusted for multiple comparison using Bonferroni-Dunn method) are indicated by **** (p < 0.0001).
