Extended Data Fig. 1: Expression profile of PsAvh413 at the transcriptional levels.
From: Phytophthora sojae boosts host trehalose accumulation to acquire carbon and initiate infection
a, Alignment of the full-length amino acid sequences of PsAvh413 in four P. sojae isolates using Clustal Omega. SP, signal peptide region; RXLR, RxLR/dEER motifs were indicated. Identical and similar residues are shaded black. Two point mutations (M1 and M2) of PsAvh413 was generated by analysis of amino acid sequences. n = 3 biologically independent samples for each treatment. b, Expression profile of PsAvh413 in susceptible soybean HC6 during P. sojae infection. Total RNA was extracted from mycelia (MY) or infected soybean roots at 0.5, 0.75, 1, 1.5, 12, 24 and 48 h post inoculation (hpi). Transcript levels of PsAvh413 were determined by qRT-PCR. The P. sojae Actin gene (VMD GeneID: 108986) was used as an internal control. c, Schematic representation of effector PsAvh413 deletion using the CRISPR-Cas9 technique. The detected primers used in PCR were shown at the positions of the forward (F) and reverse (R). d, The agarose gel shows a PCR result of PsAvh413 loci from individual P. sojae mutants. WT represents wild-type P. sojae strain P6497. M, DNA marker. e, Growth of PsAvh413-knockout mutants. Photographs were taken at 6 days after Wild-type (WT) and PsAvh413-knockout transformants incubated on 10% vegetable juice (V8) medium at 25°C. f, Colony growth was calculated based on the diameters of colonies. n = 4 biologically independent medium for each treatment. g, Western blot analysis of N. benthamiana carrying PsAvh413 and its mutant using anti-FLAG antibody. Equal loading was confirmed by Coomassie brilliant blue (CBB) staining of total proteins. Data in b and f represent the means ± s.e.m. Different letters indicate significantly different groups in f (P < 0.01, one-way ANOVA, Tukey HSD). The experiment was repeated three times with similar results.
