Fig. 3: Translational repression is broadly active against phage diversity.
From: CRISPRi-ART enables functional genomics of diverse bacteriophages using RNA-binding dCas13d
a, Network graph representation of E. coli phages and their relatives21. Nodes represent phage genomes connected by edges if they share similarity determined by vContact2 (ref. 91). Red and blue nodes represent E. coli and non-E. coli phages, respectively. Phages assessed here for dCas13d sensitivity are shaded in black. b, Anti-phage activity conferred by dCas13d when targeting an essential gene in 12 diverse phages (mean of 3 replicates), scored by EOP reduction (top) or plaque size (bottom). c, crRNA multiplexing facilitates more efficient knockdown than component guides, using phage T7 as an example. d, Transcriptome-wide knockdown screen in ssRNA phage MS2 using RBS-targeting guides. The best of two guides tested is shown. -con is a non-targeting crRNA control. dCas13d was induced as described in Methods.
