Fig. 2: DNA microbead delivery and integration into the developing in vitro and in vivo medaka retina.
From: DNA microbeads for spatio-temporally controlled morphogen release within organoids
a, Schematic illustration of medaka RO generation and time point of DNA microbead microinjection. b, Light sheet fluorescence microscopic image of a plasma membrane-stained day 3 RO post microinjection with DNA microbeads. c, Transmission image of an alive stage 20 medaka embryo 2 h after DNA microbead microinjection into its developing retina. d, Representative confocal images of whole-mount antibody-stained day 4 RO (DAPI (nuclei), Atoh7::EGFP (retinal ganglion cells), Otx2 (bipolar cells and photoreceptors) and HuC/D (amacrine and retinal ganglion cells)) after microinjection with DNA microbeads (DNA microbeads inj.; λex = 561 nm, Cy3-labelled DNA), PBS (PBS inj.) or being left uninjected (non-injection). Dashed white lines outline the DNA microbeads’ positions. Representative images from n = 25 organoids across 3 independent experiments. Scale bars, 100 µm.
