Extended Data Fig. 3: NELISA detection of CA125 based on PBAP-FGFD8 and glucose oxidase.
From: Nanopore-based enzyme-linked immunosorbent assay for cancer biomarker detection
(a) The enzymatic cleavage interaction between PBAP-FGFD8 and H2O2 derived from glucose oxidase and corresponding translocation current signal changes. (b) Quantification of NSE by NELISA using PBAP-FGFD8. Linear equation of the standard working curve: y = 16.89 lgx + 71.91; R2 = 0.993; LOD is 10 mU/mL. (c) Specificity of glucose oxidase and PBAP-FGFD8 for the detection of CA125. Different types of protein antigens (1.0 μg/mL NSE, 1.0 μg/mL SCCA, 1 kU/mL CA125, 600.0 ng/mL AFP, 500.0 ng/mL CEA and 1.0 kU/mL CA19-9) are used in the test. All data were acquired in the buffer of 3.6 M KCl, 10.0 mM PBS, pH 5.0 in trans, 1.0 M KCl, 10.0 mM PBS, pH 5.0 in cis, with the transmembrane potential held at +200 mV. Number of individual experiments n = 3. Each data comes from three independently prepared standard solution samples of the same concentration and three independent nanopore translocation experiments. Data are presented as mean ± SD.
