Extended Data Fig. 6: Schematics of the system and determination of the coefficient in the cell elongation nematic parameter Q.
From: Interplay of actin nematodynamics and anisotropic tension controls endothelial mechanics
A Schematics of the microfluidic set up showing the imposed flow rate at the channel inlet and the imposed pressure at the channel outlet. Bi Cell order parameter extracted from immunostaining of VE-cadherin QCadh as a function of the cell order parameter extracted from phase contrast brightfield QBF showing a linear correlation with a 0.5 coefficient. Bii Jump in the cell order parameter, extracted from phase contrast brightfield QBF as a function of the logarithm of the jump in radius, showing a linear correlation with a 5/2 coefficient. \({Q}_{\rm{BF}}^{+}\) and \({Q}_{\rm{BF}}^{-}\) (resp. R+ and R−) are respectively the value of QBF (resp. R) just before and just after the pressure increase.
