Extended Data Fig. 2: APVAC composition, vaccination schedule, exposure to drug and standard therapy, and course of patients.

a, Composition of personalized APVAC1 and APVAC2 drug products. b, Vaccination schedule for APVAC1 and APVAC2 and the immunomodulators. Note that APVAC1 and APVAC2 vaccinations started independently on day 15 of the first and fourth adjuvant TMZ cycle, respectively. In the default schedule, both APVACs were co-applied at the ninth APVAC1 and fourth APVAC2 vaccination, and at all four-weekly applications following the tenth APVAC1 and seventh APVAC2 vaccination. In case of delayed or failed APVAC production, alternative schedules were in place to optimally align vaccinations with standard therapy (data not shown). Any assessments at a vaccination visit, including blood draws for PBMC isolation, were performed prior to vaccinations. c, Patient exposure to study drugs. d, Exposure to standard therapy. Statistics on number of applications, dose and time lines between therapy phases are provided for all enrolled GAPVAC-101 patients (n = 16). SD, standard deviation. Reference values for the standard therapy are provided²⁹. *Completed treatment assumed with 75 mg m⁻² for 6–7 weeks calculated with 1.75-m² body surface area. **According to treatment schedule, patient data not published²⁹. e, CONSORT-like diagram for patients in the GAPVAC-101 trial. Completion for APVAC1 and APVAC2 vaccinations according to the trial protocol equals to at least 11 and 8 vaccinations, respectively. Three patients left the study before receiving APVAC2: two patients withdrew their consents; one patient experienced a grade 3 seizure (unrelated to vaccinations) and withdrew from the study. Additionally, no biomarker data for definition of APVAC2 was available for patient 4, because the tumour specimen was necrotic.