Extended Data Fig. 3: Hierarchical gating strategy used to identify ILC populations in mouse lungs.

a–c, C57BL/6 mice were aerosol infected with approximately 100 CFU Mtb and lungs were collected at different d.p.i. and flow cytometry analysis was carried out on single-cell suspensions. a, b, Flow gating strategies for ILC1s (CD45⁺CD127⁺Lin⁻NKp46⁺NK1.1⁺), ILC2s (CD45⁺CD127⁺Lin⁻NK1.1⁻Sca1⁺) and ILC3 (CD45⁺CD127⁺Lin⁻NK1.1⁻Rorγt⁺) and NKp46-expressing (CD45⁺CD127⁺Lin⁻NK1.1⁻Rorγt⁺NKp46⁺) ILC3s are shown. c, Rorγt–GFP mice were aerosol-infected with approximately 100 CFU Mtb and lungs were collected at 14 d.p.i. ILC3 (CD45⁺CD127⁺Lin⁻NK1.1⁻GFP⁺) populations were quantified using flow cytometry.