Extended Data Fig. 5: ILC3s localize within lymphoid follicles during tuberculosis.
From: Group 3 innate lymphoid cells mediate early protective immunity against tuberculosis
a, IHC staining of nuclear RORγt, CD3 and PAX5 on FFPE lung tissues from human participants with pulmonary tuberculosis or influenza (left) or macaques with latent tuberculosis infections and pulmonary tuberculosis (right). b, Representative fluorescent immunohistology scans of tuberculosis-infected human lung tissues, with CD20 (FITC), CD3 (PE–Texas Red) and CD127 (PE–Cy5). CD3−CD127+ ILCs are present adjacent to follicles (top) and granuloma-like structures (bottom). c, Cumulative total ILCs per mm2 of lung tissue (left), in histological sections from 3 different patients with tuberculosis, are increased in structures of tuberculosis histopathlogy (combined lesional tissue) in comparison to the remainder of unaffected tissue (non-lesional). However, percentages of ILCs per total cell number (DAPI+ cells) (right) are not different between regions of interest (lesional tissue, n = 31 lesions) and unaffected tissue (non-lesional, n = 3 patients). d, e, ILC3 immunofluorescence analysis of nuclear RORγt, B220 and CD3 in FFPE lung tissues from human participants with pulmonary tuberculosis (d) and macaques (e) with pulmonary tuberculosis (PTB) and latent tuberculosis infections (LTBI).
