Extended Data Fig. 5: The RIPK3 scaffold contributes to TNF-induced apoptosis in Ripk1D325A/D325A MEFs.
From: Cleavage of RIPK1 by caspase-8 is crucial for limiting apoptosis and necroptosis
a, c–e, Graphs show the percentage of primary MEFs (a, c) or BMDMs (d, e) that were viable at 24 h (a), 8 h (c, e), or 5 h (d) after treatment (−, untreated; T, TNF; N, Nec-1; i, TAK1 inhibitor 5Z-7-oxozeaenol; G, RIPK3 inhibitor GSK′843). Circles, cells from different mice (Ripk1+/+, n = 4 (a), 2 (c), and 3 (d, e); Ripk1D325A/D325A, n = 4 (a); Ripk1D325A/D325A Ripk3−/−, n = 3 (a); Ripk3−/−, n = 4 (a); Ripk1D325A/+, n = 2 (c) and 3 (d, e)). Bars, mean ± s.e.m. Unpaired two-sided t-test. ∗not determined. b, f, Western blots of primary MEFs after 4 h treatment (b) or of BMDMs (f). β-Actin loading control performed after c-FLIP (b) or p-RIPK1 (f). For gel source data, see Supplementary Fig. 1. Results representative of two independent experiments.
