Extended Data Fig. 2: Efficient metastasizers express higher levels of MCT1 than inefficient metastasizers.
From: Metabolic heterogeneity confers differences in melanoma metastatic potential
Related to Fig. 2. a, Quantification of MCT1 relative to actin bands from the western blot in Fig. 2a comparing efficient and inefficient metastasizers. b, Quantification of MCT4 relative to actin bands from the western blot in Fig. 2c comparing efficient and inefficient metastasizers. c, d, Quantification of mean fluorescence intensities for MCT1 staining in the flow cytometry plots comparing efficient (Fig. 2e) and inefficient (Fig. 2d) metastasizers. HCC15 cells and MCT1-deficient HCC15 cells were positive and negative controls (c). e, f, Immunofluorescence staining for MCT1 (green) in sections from subcutaneous tumours from inefficiently (e, UM47) or efficiently (f, M405) metastasizing melanomas. An adjacent section was stained with an antibody against the melanoma marker S100b (green). Images are representative of three independent experiments per melanoma. g, h, Immunofluorescence staining for MCT1 (green) in sections from subcutaneous tumours from inefficient (g; M498, M610 and M597) and efficient (h; M481, UT10 and M405) metastasizers. In each case, an adjacent section was stained with an antibody against S100b (green). Images are representative of results from two independent experiments per melanoma. i, j, Although efficient metastasizers often exhibited cell-surface staining (j), inefficient metastasizers typically exhibited diffuse cytoplasmic staining (i). Images are representative of results from two independent experiments per melanoma. Data are mean ± s.d. Statistical significance was assessed using Student’s t-tests (a, b, d).
