Extended Data Fig. 6: The effect of BAR deficiency on T_reg cells or T_H17 cells in gut and peripheral lymphoid organs.

a, Protein expression of VDR, FXR (also known as NR1H4) and GPBAR1 in the colonic tissue of SPF C57BL/6J mice was analysed by western blot. The red asterisks indicate the corresponding molecular weight of VDR (53 kDa), FXR (69 kDa) and GPBAR1 (33 kDa). For gel source data, see Supplementary Fig. 1. b, c, Absolute numbers of RORγ⁺Helios⁻ in the colonic FOXP3⁺CD4⁺TCRβ⁺ T_reg cell population (b) and of FOXP3⁺ T_reg cells in the CD4⁺TCRβ⁺ population (c) from mice deficient in nuclear receptors (Nr1i2^−/−Nr1i3^−/−, Nr1h3^−/−, Vdr^−/−, Nr1h4^−/− and Vdr^−/−Nr1h4^−/−) and their littermate controls. d, e, Frequencies of FOXP3⁺ in the colonic CD4⁺TCRβ⁺ cell population from mice deficient in G-protein-coupled receptors (Gpbar1^−/−, Chrm2^−/−, Chrm3^−/− and S1pr2^−/−) and their littermate controls (d) and from mice deficient in nuclear receptors (Nr1i2^−/−Nr1i3^−/−, Nr1h3^−/−, Vdr^−/−, Nr1h4^−/− and Vdr^−/−Nr1h4^−/−) and their littermate controls (e). f, g, Frequencies of RORγ⁺FOXP3⁻ in the colonic CD4⁺TCRβ⁺ cell population from mice described in d and e. h–n, T_reg cells in the spleen, mesenteric lymph node and ileum from the indicated mice were analysed. Frequencies of RORγ⁺Helios⁻ in the FOXP3⁺CD4⁺TCRβ⁺ T_reg cell population from Gpbar1^−/− (h), Chrm2^−/− (i), Chrm3^−/− (j), S1pr2^−/− (k), Nr1i2^−/−Nr1i3^−/− (l), Nr1h3^−/− (m), and Vdr^−/−, Nr1h4^−/− and Vdr^−/−Nr1h4^−/− (n) mice and their littermate controls are shown. Data are representative of two or three independent experiments in a and d–n, or are pooled from two or three independent experiments in b and c. n represents biologically independent animals. Data are mean ± s.e.m. **P < 0.01, one-way ANOVA followed by the Bonferroni post hoc test.

Source Data