Extended Data Fig. 1: Intrinsic differences between male and female B cells in forming geminal centres.
From: A GPR174–CCL21 module imparts sexual dimorphism to humoral immunity
a, Representative contour plots and frequencies of dsRed-expressing and GFP-expressing MD4-derived cells of the indicated sexes, found in the initial transfer inoculum (left panels) or in the germinal-centre compartment in draining lymph nodes five days after subcutaneous immunization (middle and right panels) of male recipient mice. b, Summary statistics of the dsRed/GFP ratio in MD4 germinal centres (GCs), normalized against the dsRed/GFP ratio in transfer inoculum. Each symbol represents one mouse (n = 17 and 16); data were pooled from three independent experiments with similar results. c, Representative distribution patterns of dsRed-expressing and GFP-expressing MD4 cells of the indicated sexes in follicles 90 h after immunization. Scale bar, 50 μm. d, Definition of the centre concentration index (CCI) and summary statistics of the CCI of dsRed+ tester cells of the indicated sexes, normalized against the CCI of GFP+ male control cells in the same follicle. Each symbol denotes one follicle (n = 38 and 46). Data pooled from two independent experiments with similar results. Scatter plots have horizontal lines denoting mean (b, d); two-tailed unpaired Student’s t-tests were used to calculate the P values at the top of each graph.
