Extended Data Fig. 3: GPR174 suppresses germinal-centre formation in male, but not female, mice.
From: A GPR174–CCL21 module imparts sexual dimorphism to humoral immunity
a, Representative cytometric profiles of FAShiGL7hi frequencies of transduced MD4 B cells in B6 recipients five days after immunization. b, c, Representative cytometric profiles of germinal centres (b) and SPPCs (c) in mice of the indicated genotypes and sexes seven days after SRBC immunization, matching the summary statistics presented in Fig. 1d, e, respectively. d, Representative cytometric profiles (left panels) and summary statistics (right panel) of germinal centres in littermate mice of the indicated genotypes and sexes, eight days after infection with LCMV Armstrong (105 plaque-forming units per mouse). The scatter plot (right) shows individual mice with lines denoting mean (n = 15, 15, 14 and 14 mice, from left to right). Data pooled from three independent experiments with similar results. A two-tailed unpaired Student’s t-test was used. e, f, Representative cytometric profiles of germinal centres (e) and SPPCs (f) in mixed bone-marrow (BM) chimeric mice of indicated types seven days after SRBC immunization, matching the summary statistics presented in Fig. 1g, h, respectively.
