Extended Data Fig. 8: Clonal expansion of T cells in the CSF.
From: Clonally expanded CD8 T cells patrol the cerebrospinal fluid in Alzheimer’s disease
a, Gating strategy for enumerating T cell subtypes in CSF from healthy elderly individuals. b, Quantification of CSF cells shows that the majority of cells are T cells, with a minority population of CD14+ monocytes and a quantifiably minor number of CD19+ B cells (n = 10 healthy subjects); mean ± s.e.m. c, Quantification of CD8+ T cell subsets shows that the majority of cells are TEM or TEMRA cells (n = 10 healthy subjects); mean ± s.e.m. d, Single-cell sorting of CSF cells shows that CD4 and CD8 T cells were sorted from control individuals and patients with AD. Data were replicated in two independent experiments. e, Donut plots depicting CSF clonality in plate-seq samples. Clones are coloured by their proportion of the total TCRαβ sequences for each subject. f, Gating strategy for drop-seq experiments. Live CSF cells were sorted using a live/dead marker. g, Multidimensional reduction and visualization by t-SNE shows distribution of CSF cells by group, sex and patient (n = 9 healthy, n = 9 MCI or AD).
