Extended Data Fig. 1: Cryo-EM structural analysis of wild-type NSD3C in complex with 187-bp NCPs.
From: Molecular basis of nucleosomal H3K36 methylation by NSD methyltransferases
a, Representative cryo-EM micrograph from a total of 2,511 micrographs of the NSD3C–NCP complex (low-pass-filtered to 20 Å). Scale bar, 100 nm. b, Selected 2D class averages of the NSD3C–NCP complex. Scale bar, 10 nm. Box size 216, pixel size 1.08 Å. c, Workflow of the image processing procedures for the NSD3C–NCP complex. It includes several rounds of 2D and 3D classification, refinement and masked refinement. The linker DNA is highly flexible, thus, it is invisible when the density map is shown in high resolution. d, Local-resolution map of the NSD3C–NCP complex final density map. e, FSC curve of the NSD3C–NCP complex final density map.
