Extended Data Fig. 4: Comparing the arrangement of common SAGA and TFIID subunits.
From: Structure of SAGA and mechanism of TBP deposition on gene promoters
a, The Taf5 NTD and Taf6 HEAT repeats have different positions in SAGA and in TFIID. The structures of Taf5 NTD, Taf6 HEAT repeats and Taf5 WD40 ___domain in SAGA (left) and in the twin lobe of TFIID19 (right) are depicted. For the sake of clarity, all other parts of SAGA and TFIID were removed. To compare the position of the Taf5 NTD and Taf6 HEAT repeats between SAGA and TFIID, both complexes were aligned to have the same orientation of the Taf5 WD40 ___domain. The top face of the WD40 repeat, which is to a large extent free in TFIID, is nearly completely engaged in SAGA. The positions of the Taf5 NTD and Taf6 HEAT repeats are defined by direct interactions of the two domains between themselves, with the WD40 surface, and with the α-helix A352–D364 of Taf5. b, SAGA and TFIID share five subunits (Taf5 and the four histone fold-containing subunits Taf6, Taf9, Taf10 and Taf12). These subunits are present as one and two copies in SAGA and TFIID, respectively. The relative positions of these subunits are depicted in both complexes. Note that in TFIID, the TBP-binding lobe was poorly resolved19,20 and therefore the second copy of only the Taf6 HEAT repeats and Taf5 NTD is shown.
