Extended Data Fig. 7: Low-dose AET promotes the differentiation of monocytic MDSCs towards macrophages in the LLC model.
From: Epigenetic therapy inhibits metastases by disrupting premetastatic niches
a, DAVID analyses of the significantly downregulated and upregulated genes using the KEGG Gene Ontology in LLC mice, treated or untreated with low-dose AET (n = 3 biological replicates). Hypergeometric test (FDR-adjusted P < 0.05). b, c, The mRNA (b) and protein (c) levels of representative transcription factors were measured by quantitative PCR and western blot, respectively. In vitro, splenic monocytic MDSCs from LLC mice were cultured for 3 days with tumour-conditioned medium. In vivo, monocytic MDSCs from both lungs of mock- (day 3) and low-dose-AET-treated LLC mice (day 3) were sorted for analysis. For gel source data, see Supplementary Fig. 1. Representative data were repeated at least three times with similar results. In b, a two-sample two-sided t-test was used, n = 3 biological replicates. All bars show mean ± s.e.m.
