Extended Data Fig. 8: Low-dose AET promotes the differentiation of monocytic MDSCs towards an interstitial macrophage-like population in the lung premetastatic microenvironment.
From: Epigenetic therapy inhibits metastases by disrupting premetastatic niches
a, Gating strategy used to identify and analyse lung interstitial macrophages in the lung premetastatic microenvironment by FACS. b, The effect of low-dose AET on lung interstitial macrophages from LLC mice. The percentage and cell counts of interstitial macrophages from both lungs in mock- and low-dose-AET-treated mice were analysed by FACS at day 3 after surgery (n = 3 mice per group). Two-sample, two-sided t-test. All bars show mean ± s.e.m. c, Gating strategy used to identify and analyse CD45.1+ lung interstitial macrophages from the lungs of recipient CD45.2 mice after the transfusion of CD45.1+ monocytic MDSCs. d, Kaplan–Meier curves showing the disease-free survival and overall survival of Ccr2-knockout LLC mice after transfusion of wild-type monocytic MDSCs (5 × 106), low-dose-AET-treated (in vivo) wild-type monocytic MDSCs (5 × 106) or vehicle at day 1 and day 4, respectively. e, Kaplan–Meier curves showing the disease-free survival and overall survival of the Ccr2-knockout LLC mice after transfusion of wild-type polymorphonuclear MDSCs (5 × 106), low-dose-AET-treated (in vivo) wild-type polymorphonuclear MDSCs (5 × 106) or vehicle at day 1 and day 4, respectively. In d, e, two-sided log-rank tests were used.
