Extended Data Fig. 11: RNA-seq analysis and RT–qPCR validation of SCDP1 patient and rescue samples.
From: Recapitulating the human segmentation clock with pluripotent stem cells
a, Heat map of gene expression levels of transcripts differentially expressed in patient cell lines SCDP1-A and SCDP1-F, when compared to wild-type (201B7) and corrected rescue clones (SCDP1-resA (A1) and SCDP1-resF (F2 and F3)). Analysis covers all stages of stepwise PSM induction and differentiation and for MESP2-knockout cell lines all stages except primitive streak. For somitic mesoderm-stage data see Fig. 4d. b, RT–qPCR-based validation of additional candidates found via RNA-seq to be upregulated in SCDP1 patient cell lines at the somitic mesoderm stage. Data are mean ± s.d. from three independent experiments.
