Extended Data Fig. 8: Cryo-EM data quality and ac4C visualization.
From: Dynamic RNA acetylation revealed by quantitative cross-evolutionary mapping
a, Surface (top) and cross-section (bottom) representations of the cryo-EM density maps coloured according to local resolution distribution. Growth conditions and T. kodakarensis strains used in the study along with PDB and EMDB accession codes are indicated. Resolution values are colour-coded according to the right index and are presented in Å. b, Model in density for multiple ac4C positions in wild-type T. kodakaresis grown at 85 °C (orange) and 65 °C (yellow) compared to an ac4C ΔTkNAT10 strain (mutant, blue) indicating the absence of acetate density (highlighted in light orange) in the mutant and in multiple positions of the strain grown at 65 °C. Positions highlighted with an asterisk are also acetylated in the 65 °C strain whereas positions that are unmarked are acetylated only in the archaea grown at 85 °C. These data are in good agreement with both the genomic sequencing and MS approaches described in this manuscript, that similarly indicate that ac4C distribution is highly dependent on growth temperature. A detailed list of ac4C distribution and comparison with other methods is provided in Supplementary Table 4. For a 2D map with ac4C distribution, see Extended Data Fig. 9d.
