Extended Data Fig. 6: Analysis of TRPA1 tail currents.
From: Irritant-evoked activation and calcium modulation of the TRPA1 receptor
a, Scaled averaged basal (WT, n = 10 independent experiments; C665S, n = 6), IA (100 μM; WT, n = 5; C665S, n = 5), or BIA (100 μM; C665S, n = 6)-evoked tail currents for TRPA1 WT and C665S mutant channels. Mean deactivation time constants (τ) are shown with 95% CI in parentheses. Scale bar, x, 5 ms; y, arbitrary units. Data were acquired in whole-cell patch-clamp mode after a 500-ms pre-pulses (−80 to 80 mV in 10 mV increments) followed by a 250-ms test pulse (−120 mV). Vhold = −80 mV. b, c, Quantification of changes in IA (b) and BIA (c) -evoked TRPA1 tail-current decay time constants in WT and C665S TRPA1. Statistical significance is represented as the results of a ratio paired two-tailed Student’s t-test; in b, *P = 0.01; in c, *P = 0.02, **P = 0.009.
