Extended Data Fig. 6: Cell number and IF relative to dose–response orthogonal validation in Vero E6 cells.

a, Vero E6 cells were pre-treated for 16 h with increasing concentrations of the indicated compound and then infected with SARS-CoV-2 with MOI = 0.1. 24 h post-infection, cells were fixed, and immunostained, and imaged. For each condition, the total amount of cells stained with DAPI was calculated. Data are normalized to the average of DMSO-treated wells. The heat map represents the normalized cell number of the indicated 21 compounds in dose–response, on a scale from 0 to 1, on the average of five independent experiments. Compounds are grouped in predicted functional clusters. Concentrations are rounded. Corresponding antiviral activities of these compounds are shown in Fig. 3a. b, Representative immunofluorescence images corresponding to one of the three dose–responses illustrated in Fig. 3. For each condition, the corresponding entire well is shown (4x objective). Scale bar = 1.35 mm. c, Dose–response curves for additional antiviral compounds. Vero E6 cells were pre-treated for 16 h with increasing concentrations of the indicated compound and then infected with SARS-CoV-2 with MOI = 0.1 in the presence of the compound. 24 h post-infection, cells were fixed, and an immunofluorescence was performed. For each condition, the percentage of infection was calculated as the ratio between the number of infected cells stained for CoV NP and the total amount of cells stained with DAPI. Dose–response curves for both infectivity (black) and cell number (red) are shown. Data are normalized to the average of DMSO-treated wells and represent mean ± s.e.m. for n = 5 independent experiments.