Extended Data Fig. 9: Inositol trisphosphate counters butyrate-induced inhibition of colonoid growth.
From: Microbiota-derived metabolite promotes HDAC3 activity in the gut
a, b, Growth of colonoids generated from GF mice and treated with vehicle or InsP3 (IP3) (a) (10nM; n = 60 colonoids/treatment; *P = 0.029) or phytate vs phytase-digested phytate (b) (0.2 mg/ml; n = 40 colonoids/treatment; **P = 0.0012). c, Representative images of GF colonoids treated with vehicle or 1mM butyrate, scale bars, 100 μm. d, e, Representative images (d) (red: phalloidin, blue: DAPI), scale bars, 100 μm and growth (e) of colonoids from GF mice treated with vehicle, 5 mM butyrate, or 5 mM butyrate + 50 nM InsP3; n = 30 colonoids/treatment; *P = 0.0409, ***P = 0.0001). All graphs are mean of biological replicates ± s.e.m.; unpaired two-tailed t test. Data were independently repeated two (b) or four (a, c–e) times with similar results. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001.
