Extended Data Fig. 7: Non-centrosomal PCM foci nucleate microtubules and contribute to spindle assembly in RPE-1 cells.
From: Targeting TRIM37-driven centrosome dysfunction in 17q23-amplified breast cancer
Related to Fig. 3. a, Representative time-lapse images of mitosis in DMSO-treated control PLK4AS TP53−/− RPE-1 cells. n = 3 biological replicates. Scale bar, 5 μm. b, Representative time-lapse images of PCM foci formation during mitosis in acentrosomal PLK4AS TP53−/− RPE-1 cells. n = 3 biological replicates. Scale bar, 5 μm. Arrows indicate PCM foci. c, Representative time-lapse images of microtubule nucleation from centrosomes in the mitotic spindle of DMSO-treated control PLK4AS TP53−/− RPE-1 cells. n = 3 biological replicates. Scale bars, 5 μm. d, Representative time-lapse images of microtubule nucleation from PCM foci incorporated into the mitotic spindle of acentrosomal PLK4AS TP53−/− RPE-1 cells. n = 3 biological replicates. Scale bar, 5 μm. e, Representative time-lapse images of microtubule nucleation from a PCM focus before its incorporation into the mitotic spindle in acentrosomal PLK4AS TP53−/− RPE-1. n = 3 biological replicates. Scale bar, 1 μm.
