Extended Data Fig. 10: TRIM37 overexpression delays centrosome maturation in G2/M phase.
From: Targeting TRIM37-driven centrosome dysfunction in 17q23-amplified breast cancer
Related to Fig. 4. a, Quantification of centrosomal α-tubulin intensity from time-lapse movies of dividing MCF-7 cells expressing either control vector or TRIM37-targeting shRNA. Quantification of >20 cells. Mean ± s.e.m. b, Quantification of the distance between the two centrosomes at NEBD in MCF-7 cells. n = 3 biological replicates, each comprising >12 cells. P values, unpaired two-tailed t-test. Mean ± s.e.m. c, Quantification of centrosomal α-tubulin intensity from time-lapse movies of dividing RPE-1 tet-on TRIM37 cells. Quantification of >20 cells. Mean ± s.e.m. d, Representative time-lapse images of centrosome maturation in MCF-7 cells. n = 3 biological replicates. Scale bars, 5 μm. e, Representative time-lapse images of centrosome maturation in RPE-1 tet-on TRIM37 cells. n = 3 biological replicates. Scale bars, 5 μm.
